Essential Amino Acids

María-Concepción Aristoy and Fidel Toldrá Contents

22.1 Introduction 385

22.2 Sample Preparation 388

22.2.1 Free Essential Amino Acids 388

22.2.2 Total Essential Amino Acids 388

22.2.2.1 Acid Hydrolysis 388

22.2.2.2 Alkaline Hydrolysis 390

22.2.2.3 Enzymatic Hydrolysis 390

22.3 Analysis 391

22.3.1 Direct Spectrophotometric Methods 391

22.3.2 Chromatographic Methods 391

22.3.2.1 Derivatization 391

22.3.2.2 Separation and Detection 392

References 394

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