Essential Amino Acids

María-Concepción Aristoy and Fidel Toldrá Contents

22.1 Introduction 385

22.2 Sample Preparation 388

22.2.1 Free Essential Amino Acids 388

22.2.2 Total Essential Amino Acids 388 Acid Hydrolysis 388 Alkaline Hydrolysis 390 Enzymatic Hydrolysis 390

22.3 Analysis 391

22.3.1 Direct Spectrophotometric Methods 391

22.3.2 Chromatographic Methods 391 Derivatization 391 Separation and Detection 392

References 394

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