Dipeptidyl Peptidase Iii Ec 34144

Contrary to DPP I, reducing agents such as cysteine, DTT, and mercaptoethanol (4 mM) cause a strong inhibition of DPP III activity (Figure 7.3); thus, their use must be avoided in reaction buffers. This enzyme is also inhibited by chelating agents such as o-phenanthroline (0.8 mM), EDTA and EGTA (4 mM); 3/-chloromercuribenzoate and DTNB (0.05 mM); 4-DCI (0.05 mM), Hg2+ (0.05 mM), and Cd2+ (0.2 mM), and the dipeptide Tyr-Tyr (0.4 mM). DPP III is activated by Co2+ (0.05 mM). The enzyme is sensitive to temperature and is inactivated in just 30 min when incubated at 55°C.

Assay techniques, based on color, fluorescence, or peptide determination, are the same as for DPP I, except pH 8, the presence of 0.05 mM Co2+, and the absence of any reducing agent in the reaction buffer (Table 7.3).

Was this article helpful?

0 0
Lower Your Cholesterol In Just 33 Days

Lower Your Cholesterol In Just 33 Days

Discover secrets, myths, truths, lies and strategies for dealing effectively with cholesterol, now and forever! Uncover techniques, remedies and alternative for lowering your cholesterol quickly and significantly in just ONE MONTH! Find insights into the screenings, meanings and numbers involved in lowering cholesterol and the implications, consideration it has for your lifestyle and future!

Get My Free Ebook

Post a comment