Methods of Cholesterol Determination in Food

Various methods have been developed for cholesterol determination in foods due to the necessity of precise and accurate results. The majority of the methods used in food are gravimetric, colori-metric, enzymatic, and chromatographic. The first method used to determine cholesterol in foods was the gravimetric method, in which the cholesterol is separated by precipitation with digitonin or tomatin.5 This analytical method is time consuming and has low sensitivity. Although colori-metric methods...