11. Muscle cell contracts

7B. ACh inactivated by acetylcholinesterase into a cell that does not normally make ACh-activated channels (such as the egg cell of a frog), the cell's machinery of protein synthesis will read the message and make functional ACh-activated channels. The properties of these channels can then be examined using patch-clamp recording. Thus, by altering the mRNA, experimenters can make discrete changes in the channel protein and then see how the change affects the behavior of the channel. For example, in this way the parts of each subunit that probably make up the ion-conducting pore have been identified.

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